Reproducible extracellular matrices for tumor organoid culture: challenges and opportunities.

Authors

Kan Li, Yibo He, Xue Jin, Ketao Jin, Jun Qian

Published in

Journal of translational medicine. Volume 23. Issue 1. Pages 497. May 01, 2025. Epub May 01, 2025.

Abstract

Tumor organoid models have emerged as valuable 3D in vitro systems to study cancer behavior in a physiologically relevant environment. The composition and architecture of the extracellular matrix (ECM) play critical roles in tumor organoid culture by influencing the tumor microenvironment and tumor behavior. Traditional matrices such as Matrigel and collagen, have been widely used, but their batch-to-batch variability and limited tunability hinder their reproducibility and broader applications. To address these challenges, researchers have turned to synthetic/engineered matrices and biopolymer-based matrices, which offer precise tunability, reproducibility, and chemically defined compositions. However, these matrices also present challenges of their own. In this review, we explore the significance of ECMs in tumor organoid culture, discuss the limitations of commonly used matrices, and highlight recent advancements in engineered/synthetic matrices for improved tumor organoid modeling.

PMID:
40312683
Bibliographic data and abstract were imported from PubMed on 02 May 2025.

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