Isolation of xylanase-producing yeast Pseudozyma tsukubaensis from chrysanthemum.

Authors

Jeong-Ah Yoon, Eun-Hee Park, Yi-Yong Yoon, Myoung-Dong Kim

Published in

Food science and biotechnology. Volume 34. Issue 10. Pages 2217-2224. Epub Mar 01, 2025.

Abstract

Yeast strains showing xylanase activity were explored from various natural sources, such as flowers and soil. A yeast strain showing high xylanase activity on the Remazol Brilliant Blue-xylan plate was isolated from the flower of yellow chrysanthemum and identified as Pseudozyma tsukubaensis through analysis of the nucleotide sequence of the internal transcribed spacer region of 18S ribosomal RNA. P. tsukubaensis MBY/L1662 exhibited higher tolerance to fermentation inhibitors than the control strain. The maximum xylanase activity of 3,244.11 ± 210.01 U/mg protein was obtained for P. tsukubaensis MBY/L1662 grown at pH 5 and 10 g/L xylose. The ability of P. tsukubaensis MBY/L1662 to degrade xylan will be a critical stepping stone for its various applications.

PMID:
40351730
Bibliographic data and abstract were imported from PubMed on 12 May 2025.

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