Authors
Wenxia Song, Xianqin Lu, Xiaolong Han, Yinbo Qu
Published in
Applied biochemistry and biotechnology. May 15, 2025. Epub May 15, 2025.
Abstract
Cellulase cocktails play a crucial role in enzymatic saccharification of lignocellulosic biomass, a critical step in sustainable biofuel production. However, the efficiency of cellulase cocktails remains suboptimal due to incomplete understanding of enzyme synergy. In this study, we aimed to enhance biomass hydrolysis by optimizing the synergistic action of recombinant Cel5B, an endoglucanase and Cel7A-2, cellobiohydrolase from Penicillium oxalicum within a reconstituted cellulase system. Both enzymes were successfully expressed, purified, and characterized to optimize their synergistic action. The enzymatic properties of both enzymes were assessed, revealing optimal activities at 55-65°C and pH 4.2-4.8. Their combined action significantly enhanced the hydrolysis of filter paper and corncob residues. Using a central composite design, enzyme ratios were optimized to 11.6% rCel7A-2, 23.7% rCel5B, and 31.7% β-glucosidase. This formulation achieved the predicted glucan conversion of 58.1% for corncob residue hydrolysis, with experimental validation yielding 57.2%, demonstrating a 98.5% agreement with the model. Compared to the that of the commercial cellulase produced from P. oxalicum (38.1%), the optimized process improved glucan conversion by 50.1%. These findings demonstrate the effectiveness of rational enzyme synergy optimization and provide valuable insights into the strategy for improving the lignocellulosic biomass saccharification efficiency of the cellulase system.
PMID:
40372654
Bibliographic data and abstract were imported from PubMed on 15 May 2025.
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