Plasmid DNA Cleavage Assay with Eukaryotic Topoisomerase II.

Authors

Allison J Thomas, Brooke D Latham, Addison K O'Brian, Mattalyn R Hardin, Joseph Deweese

Published in

Methods in molecular biology (Clifton, N.J.). Volume 2928. Pages 89-95.

Abstract

Measuring DNA cleavage levels has been a long-standing method in the field of topoisomerases. Due to the formation of a reversible covalent enzyme:DNA linkage, topoisomerase II activity can be monitored under varying conditions by means of measuring single- and double-stranded DNA breaks. This chapter will provide a method for measuring plasmid DNA cleavage levels generated by eukaryotic topoisomerase II with a specific focus on human type IIA topoisomerases. This method can be utilized to perform reactions as single timepoint, time course, and drug titrations.

PMID:
40372639
Bibliographic data and abstract were imported from PubMed on 15 May 2025.

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