A Thermus thermophilus Argonaute (TtAgo) cleavage-aided isothermal amplification strategy (TAC-IAS) for precise detection of locus-specific DNA methylation.

Authors

Fangfang Sun, Bingjie Han, Yi Zhao, Xing Chen, Hui Tian, Chenghui Liu, Yuanyuan Sun

Published in

Chemical communications (Cambridge, England). May 23, 2025. Epub May 23, 2025.

Abstract

A versatile TtAgo cleavage-aided isothermal amplification strategy (TAC-IAS) is devised for precise detection of DNA methylation with high flexibility and universality compared to methylation-restriction endonuclease, reliably identifying methylation with an abundance of 0.1% in a large pool of unmethylated fragments.

PMID:
40406800
Bibliographic data and abstract were imported from PubMed on 23 May 2025.

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