Bioconversion of C1-gases by mixotrophic co-cultures fermentation with C. carboxidivorans and C. beijerinkii.

Authors

Marina Fernández-Delgado, Pedro Enrique Plaza, M Teresa García-Cubero, Susana Lucas, Mónica Coca, Juan Carlos López-Linares

Published in

Bioresources and bioprocessing. Volume 12. Issue 1. Pages 45. May 26, 2025. Epub May 26, 2025.

Abstract

The development of newfangled bioprocess strategies for the capture of C1-gases (CO and CO₂) and their bioconversion into valuable products is currently one of the main focuses of research in order to achieve a more resilient world. This work analyses the viability of the co-culture C. carboxidivorans and C. beijerinkii to produce bioproducts (bioalcohols and organic acids) in mixotrophic conditions. In this way, the bioconversion of C1 gases (CO and CO₂), in the presence of Fe⁰, using mixotrophic co-culture fermentation by C. carboxidivorans and C. beijerinkii, was evaluated, analyzing the influence of the ratio between both microorganisms, the pH, and the presence of Fe⁰. As a result, up to 7 g/L of butanol were achieved at pH 7, 12.5 g/L Fe⁰, and using a 1:1 ratio of C. carboxidivorans: C. beijerinkii, also improving the production of ethanol, acetic acid, and butyric acid as compared to individual culture fermentations. Finally, the operation in a bioreactor, comparing discontinuous and continuous gas feeding operation modes, was also studied, with better C1-gases utilization and overall fermentation efficiency (7 vs 4.6 g/L butanol) in continuous gas operation mode.

PMID:
40415020
Bibliographic data and abstract were imported from PubMed on 26 May 2025.

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