Development of microsatellite markers for Leiarius marmoratus obtained by partial de novo genome assembly.

Authors

Dárcia Gabriela B DA Costa, Daralyns B Macedo, Carlos Willian D Dantas, Regianne M Dos Santos Correa, Ádria D Dos Santos, Hendrya Julianny P Coelho, Carla Giovanni Á Moreira, Heden Luiz M Moreira, Rommel Thiago J Ramos, Marília Danyelle N Rodrigues

Published in

Anais da Academia Brasileira de Ciencias. Volume 97Suppl 2. Issue Suppl 2. Pages e20241094. Epub May 30, 2025.

Abstract

This study addresses the identification of repetitive regions called microsatellites in the genome of the Amazonian species Leiarius marmoratus. An efficient methodology was used with sequencing and genome assembly to obtain and map specific markers. The programs MISA and EasySSR identified 221,273 and 145,601 markers, respectively. In addition, primers were developed, of which 590 were considered successful, according to in-silico testing with the Batchprime3 program. The results confirm the quality of the methodology, which can be applied in studies of genetic improvement, variability control, and genomic and comparative analyses.

PMID:
40465940
Bibliographic data and abstract were imported from PubMed on 05 Jun 2025.

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