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Chronic exposure to diquat-based herbicide induces morphological, behavioral, and growth alterations in Physalaemus cuvieri tadpoles.

Created on 27 Jun 2025

Authors

Diana Paula Giacomel, Guilherme Felicioni, Bruna Macedo Simoes Sergio, Aline Pompermaier, Wagner Antonio Tamagno, Paulo Afonso Hartmann, Marilia Hartmann

Published in

Ecotoxicology (London, England). Jun 27, 2025. Epub Jun 27, 2025.

Abstract

Pesticides pose a serious threat to non-target organisms and are classified as key contributors to amphibian population decline. Amphibians, known for their sensitivity to environmental changes, serve as valuable bioindicators of environmental health. In this study, we assessed the toxicity of a diquat-based herbicide (DBH) on the bioindicator species Physalaemus cuvieri. Tadpoles were exposed to a broad toxicity gradient of DBH (20, 50, 250, 500, 1000, and 1500 µg L-1) for 336 h. Survival, body mass index (BMI), scaled mass index (SMI), malformations, and swimming activity were evaluated. Exposure to 1000 and 1500 µg L-1 significantly reduced survival. Interestingly, concentrations of 20, 50, 1000, and 1500 µg L-1 led to increases in BMI and SMI. Malformations and alterations in swimming behavior were detected at all concentrations. These findings demonstrate that DBH is highly toxic to P. cuvieri tadpoles, severely impairing their survival, physiology, and behavior. These results have significant environmental implications, as tadpoles play a crucial role in aquatic ecosystems, and this sensitivity to DBH can trigger imbalances in trophic dynamics. Furthermore, amphibians are bioindicators, and the impacts observed here reinforce the need for rigorous monitoring of potentially toxic chemicals in aquatic ecosystems to preserve biodiversity, especially among non-target species.

PMID:
40576887
Bibliographic data and abstract were imported from PubMed on 27 Jun 2025.

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