Mo₂B₂/In₂S₃ coupled with Y-shaped DNA amplification for photoelectrochemical nucleic acid assay.

Authors

Huiyu Gao, Zunchao Wei, Wenhao Guo, Dan Chen, Xu Hun

Published in

Mikrochimica acta. Volume 192. Issue 7. Pages 461. Jun 27, 2025. Epub Jun 27, 2025.

Abstract

MBene is a 2D material stripped from a ternary transition metal boride (MAB). In recent years, attention has gradually shifted from the stripping of MBene to its application. Here, we prepared 2D Mo₂B₂ by treating HCl/H₂O₂ solution for MoAlB, and Mo₂B₂/In₂S₃ photoelectroactive material was fabricated by hydrothermal method to construct a photoelectrochemical (PEC) assay for dengue fever virus DNA (DENVD) detection. Hairpin DNA1 (H₁) was linked to the electrode surface by the Au-S bond. In the presence of DENVD, it opened the end of H₁ and introduced Fc-modified hairpin DNA2 (H₂) and hairpin DNA3 (H₃) onto the electrode surface. The Y-shaped DNA was formed by catalytic hairpin assembly (CHA) reaction, and Fc enhances the PEC signal. Fc is oxidized to Fc⁺ to provide electrons for holes, reducing the combination of electrons and holes of Mo₂B₂/In₂S₃, and enhancing the PEC response. The PEC assay had a detection range of 1.0 aM-1.0 fM and provided a low detection limit of 0.5 aM. Moreover, it also has good specificity, reproducibility, and stability indicating its promising potential in clinical diagnosis. In addition, this study provides a new idea for the application of 2D MBene materials.

PMID:
40576861
Bibliographic data and abstract were imported from PubMed on 27 Jun 2025.

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