Authors
Rocío Paz-González, Patricia Quaranta, Selva Riva-Mendoza, Pablo Domínguez-Guerrero, Francisco J Blanco García, Cristina Ruiz-Romero, Valentina Calamia, Lucía Lourido
Published in
Methods in molecular biology (Clifton, N.J.). Volume 2929. Pages 97-106.
Abstract
Multiplex antibody microarray assays based on suspension microsphere technology have emerged as a powerful tool in both basic and translational biomedical research, enabling the simultaneous quantification of multiple proteins within a single assay. Developing custom-designed multiplex assays offers the advantage of flexibility beyond predefined biomarker panels; nonetheless, addressing cross-reactivity remains a crucial step to ensure assay reliability and accuracy results. This chapter provides a comprehensive, step-by-step methodology for evaluating cross-reactivity in multiplex antibody microarrays, detailing techniques to validate and optimize assay performance. Additionally, it explores strategies for mitigating cross-reactivity to enhance the precision and interpretability of assay results.
PMID:
40601146
Bibliographic data and abstract were imported from PubMed on 02 Jul 2025.
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