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Pepper JAZ Protein CaJAZ1-06 Negatively Regulates Drought Stress and Abscisic Acid Signaling.

Created on 14 Jul 2025

Authors

Junyoung Cho, Chae Woo Lim, Dae Sung Kim, Sung Chul Lee

Published in

Physiologia plantarum. Volume 177. Issue 4. Pages e70390.

Abstract

JASMONATE ZIM-DOMAIN (JAZ) proteins are well-known key JA signaling repressors that play a crucial role in regulating various abiotic stresses, such as drought, salinity, and cold. However, their functional involvement in response to drought stress in pepper plants remains elusive. Here, we isolated a novel JAZ protein, namely, CaJAZ1-06 (Capsicum annuum JAZ1 from chromosome 06), from pepper plants and analyzed the expression of CaJAZ1-06 under drought stress conditions. Our results showed that its expression significantly increased compared with that of other pepper JAZ proteins, including its homologous gene CaJAZ1-03, a negative regulator of drought stress and ABA signaling. We also investigated the biological role of CaJAZ1-06 by silencing its expression in pepper plants through virus-induced gene silencing and by overexpressing it in Arabidopsis thaliana transgenic plants. In response to drought stress, CaJAZ1-06-silenced pepper plants showed enhanced tolerance, accompanied by a reduced transpirational water loss and increased expression of stress-responsive genes compared with the control plants. Conversely, the CaJAZ1-06 overexpressing Arabidopsis plants exhibited reduced drought tolerance, with increased transpirational water loss relative to the control plants. Furthermore, CaJAZ1-06 overexpression resulted in ABA insensitivity during seedling and adult stages. Therefore, our findings suggest that CaJAZ1-06 acts as a negative regulator of drought stress responses and ABA signaling.

PMID:
40654046
Bibliographic data and abstract were imported from PubMed on 14 Jul 2025.

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