[Studies on the expression and function of the PIWI-interacting RNA piRNA-27619 in prostate cancer with a focus on its potential oncogenic role].

Authors

Jenny Mauch, Hubert Kübler, Burkhard Gerhard Kneitz

Published in

Aktuelle Urologie. Jul 22, 2025. Epub Jul 22, 2025.

Abstract

Prostate cancer is the most common malignancy in men, yet predictive biomarkers for assessing individual risk remain insufficient. Recent research has highlighted PIWI-interacting RNA 26719 (piRNA-26719) as a potential tumor-associated gene 1 2. This study aims to identify and characterize the expression and function of piRNA-26719 in prostate cancer.The expression of piRNA-26719 was analyzed in a high-risk prostate cancer cohort (n=121, PSA preoperative >20ng/ml) and prostate cancer cell lines using quantitative RT-PCR. Functional analyses, including RNA interference (RNAi) and subsequent assays for cell proliferation, apoptosis, and cell cycle distribution, were conducted in prostate cancer cell lines.piRNA-26719 was biochemically validated. Expression analysis revealed a trend toward upregulation of piRNA-26719 in primary high-risk prostate tumors compared to benign hyperplasia, with significant overexpression in lymph node metastases. Functional studies demonstrated that inhibition of piRNA-26719 led to decreased proliferation in prostate cancer cells. Apoptosis assays confirmed the induced activation of caspase-dependent apoptosis, and cell cycle analysis indicated S-phase arrest in piRNA-26719-inhibited cells, but not in the less malignant 22RV1 line.These findings suggest an oncogenic role for piRNA-26719 in high-risk prostate cancer, particularly in aggressive forms, as evidenced by its overexpression in metastatic tissues. The oncogenic function of piRNA-26719 in prostate cancer is supported by its critical role in cellular proliferation, apoptosis, and cell cycle progression. These results warrant further investigation into piRNA-26719's potential as a prognostic biomarker and therapeutic target in prostate cancer management.

PMID:
40695484
Bibliographic data and abstract were imported from PubMed on 23 Jul 2025.

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