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Salivary and serum biomarkers profile of patients with rheumatoid arthritis and temporomandibular disorders.

Created on 01 Aug 2025

Authors

M Dozet, V Fugosic Mesic, A M Laskarin, V Drvar, R Grzic, S Spalj

Published in

Clinical rheumatology. Aug 01, 2025. Epub Aug 01, 2025.

Abstract

The objective was to analyze the association of biomarkers with temporomandibular disorders (TMDs) and systemic inflammation in patients with rheumatoid arthritis (RA).
The sample included 60 subjects aged 32-65 (82% females), with 30 having RA. Saliva and serum samples were analyzed for interleukin-4 (IL-4), IL-18, and interferon-gamma (IFN-γ). Serum was additionally analyzed for calcium, magnesium, phosphate, urates, alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT). An international diagnostic protocol for TMDs was used.
Subjects with RA had higher salivary IL-4, serum IFN-γ/IL-4 ratio, GGT level, and lower calcium (p ≤ 0.015). Correlations between salivary cytokines and matching serum cytokines were significant only for IFN-γ (r = 0.348; p = 0.008). Somatization and depression correlated with serum IFN-γ/IL-4 ratio (r = 0.447-0.551; p ≤ 0.012). TMD was more prevalent in RA than in controls (43 vs. 27%). Subjects with RA had a higher orofacial pain intensity, somatization, and depression than those without RA (p ≤ 0.018).
Salivary and serum biomarkers were not related to the presence of TMDs in RA. Saliva showed a low potential to reveal the ongoing pathology of RA or TMD. Increased IL-4 and decreased calcium imply control of RA by medications, while increased GGT is linked to systemic inflammation in RA. Key Points • Serum biomarkers more accurately reflect the underlying pathology of RA and TMD than salivary biomarkers. • No specific diagnostic biomarkers were identified that distinguish RA patients with TMDs from those without TMDs.

PMID:
40748559
Bibliographic data and abstract were imported from PubMed on 01 Aug 2025.

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