Authors
Jiushi Liu, Ruilin Xiong, Leijiao Liu, Tai-Ping Zhou, Jiayu Xue, Dan Sun, Ranran Gao, Shanshan Chen, Rui Xu, Yuanyuan Xing, Shuang Peng, Bin Li, Xueping Wei, Wei Sun, Xiwen Li, Jun Ai, Hongmei Luo, Bengang Zhang, Binju Wang, Zhichao Xu, Haitao Liu
Published in
Science advances. Volume 11. Issue 33. Pages eadw0486. Aug 15, 2025. Epub Aug 15, 2025.
Abstract
Schisandraceae, an early-diverging angiosperm lineage, produces dibenzocyclooctadiene (DBCOD) lignans, unique bioactive compounds with liver-protecting properties. Although DBCOD lignan chemodiversity is well documented, their biosynthesis and evolution remain unclear. Here, we present a high-quality Schisandra chinensis genome, completing genomic representation of early angiosperms. Phylogenetic analysis confirms Austrobaileyales as sister to monocots, magnoliids, and eudicots, clarifying their evolutionary position. We identified an Austrobaileyales-specific whole-genome duplication postdiversification from Amborellales and Nymphaeales. Integrating coexpression networks and biochemical assays, we delineated five key steps in DBCOD lignan biosynthesis, including dimerization, hydroxylation, methylation, and C─C phenol coupling. Notably, we found SchCYP719G1b, a previously unidentified enzyme catalyzing C─C linkage to form the signature DBCOD scaffold. Substrate selectivity assays and quantum mechanical/molecular mechanics calculations suggested that the para-hydroxyl diradical electronic properties preferentially drive selective C2─C2' coupling over alternative pathways. Our findings illuminate early angiosperm evolution and the molecular basis of specialized lignan diversity.
PMID:
40815645
Bibliographic data and abstract were imported from PubMed on 16 Aug 2025.
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