Recombinant Escherichia coli biotransformation of biliverdin to synthesize bilirubin.

Authors

Yue Zhang, Haoni Luan, Wenhan Qiu, Wei Xu, Wei Feng, Fei Wang, Peng Song

Published in

World journal of microbiology & biotechnology. Volume 41. Issue 9. Pages 315. Aug 26, 2025. Epub Aug 26, 2025.

Abstract

Bilirubin, a heme-derived metabolite with pronounced bioactivity, exhibits significant potential for pharmaceutical applications. This study developed a recombinant Escherichia coli-based biliverdin biotransformation system, enabling efficient bilirubin synthesis via heterologous expression of a novel biliverdin reductase (BvdR', XP_032760038.1) from Rattus norvegicus and a glucose dehydrogenase (GDH) from Priestia megaterium. Under optimized conditions (520 mg/L biliverdin, pH 7.5, 37 °C), the system achieved a biliverdin conversion rate of 99.2% and a bilirubin yield of 89.9%. The integration of an NADPH in situ regeneration system significantly reduced synthesis costs, providing a novel strategy for scalable bilirubin production.

PMID:
40856945
Bibliographic data and abstract were imported from PubMed on 26 Aug 2025.

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