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The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate-Protein Interactions.

Created on 29 Aug 2025

Authors

Kristina Sturm, Michael Hothorn

Published in

Methods in molecular biology (Clifton, N.J.). Volume 2972. Pages 153-169.

Abstract

Grating-coupled interferometry (GCI) is a biophysical method suitable to studying the binding kinetics of protein-ligand interactions. Here, we describe the application of biotinylated inositol pyrophosphate (PP-InsP) nutrient messengers to characterize PP-InsP-protein interactions using a Creoptix WAVEsystem.

PMID:
40879985
Bibliographic data and abstract were imported from PubMed on 29 Aug 2025.

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