Authors
Man Gong, Lingling Zhou, Liurunzi Xv, Meng Wang, Qingli Yang, Xiudan Hou
Published in
Mikrochimica acta. Volume 192. Issue 10. Pages 645. Sep 08, 2025. Epub Sep 08, 2025.
Abstract
As the most dangerous mycotoxin, aflatoxin B1 (AFB1) has caused some food safety issues to be concerned. In this study, a simultaneous detection and degradation method towards AFB1 was established. Covalent-organic frameworks (COFs) were firstly synthesized and directly in situ deposited on the stainless-steel mesh, which would trigger the free-radical polymerization of acrylamide to form a hydrogel coating. The synthesized Pt@Au nanoparticles as a colorimetric signal probe exhibited the high peroxidase catalytic activity (Km = 0.14 mM, Vmax = 4.57 × 10-8 mol (L s)-1, Kcat = 1.40 × 106 s-1). Coupled with a smartphone application, the prepared "sandwich-structure" colorimetric sensor enables detection of AFB1 with a desirable linear range (0.4-200 μg L-1) and a low detection limit (0.085 μg L-1). The porous channel of the COF-based hydrogel prevents the interference of other macromolecules due to the size effect, resulting in a desirable matrix effect of 92.09-104.71%. The recoveries spiked with different concentrations of AFB1 were 93.75-132.10%. The degradation efficiency of AFB1 could reach 91.74% within 180 min. Through the investigation using ESI-MS, four degraded intermediate products were identified to form the possible degraded pathway. Overall, the fabrication of COFs-based sensing platform provided a new approach to detect and degrade mycotoxins.
PMID:
40921876
Bibliographic data and abstract were imported from PubMed on 09 Sep 2025.
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