Effects of low omega-6/omega-3 ratio and high omega-9/omega-6 ratio oil blend on inflammation and oxidative stress after tooth extraction in rats.

Authors

Radamés Bezerra Melo, Gabriella Alves Julião Costa, Paulo Goberlânio de Barros Silva, Reinaldo Barreto Oriá, Cristhyane Costa de Aquino, Paulo Roberto Leitão Vasconcelos

Published in

Inflammopharmacology. Sep 09, 2025. Epub Sep 09, 2025.

Abstract

This study evaluated the effects and mechanisms of antioxidant and anti-inflammatory oils with a high omega-9:omega-6 ratio and a low omega-6:omega-3 ratio on post-extraction healing in rats.
A total of 128 Wistar rats were divided into four groups: Sham, Saline, Isolipidic, and Anti-inflammatory/Antioxidant. The animals received one of the following treatments: (1) 0.9% NaCl solution (Sham Group or Saline Group); (2) an isolipidic mixture containing alpha-linolenic acid (ALA) and a combination of ω-9, ω-6, and ω-3 fatty acids derived from corn oil and soybean oil-rich in omega-6:omega-3 and omega-9:omega-6 ratios (Isolipidic Group); or (3) an anti-inflammatory and antioxidant mixture containing ALA, docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), composed of olive oil (61%), canola oil (24%), and fish oil (15%) (Test Group). Treatments were administered by gavage for four days pre-extraction and three days post-surgery. Animals were euthanized at 3, 7, 14, and 21 days post-extraction. Blood and tissue samples were analyzed for myeloperoxidase, osteoclast count, IL-1β, NFκB, iNOS, BMP-2 expression, glutathione (GSH), and thiobarbituric acid reactive substances (TBARS).
The Anti-inflammatory/Antioxidant mixture significantly reduced myeloperoxidase expression, osteoclast count, GSH, TBARS, and IL-1β levels on day 7. It also decreased NFκB, iNOS, and BMP-2 expression on days 3 and 7 compared to the Saline and Isolipidic groups.
The Anti-inflammatory and Antioxidant mixture effectively reduced inflammation and oxidative stress during the inflammatory and proliferative phases of post-extraction healing, suggesting potential benefits in clinical applications for oral surgery recovery.

PMID:
40924306
Bibliographic data and abstract were imported from PubMed on 09 Sep 2025.

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