Decoding Tupiperla illiesi Froehlich 1998 (Plecoptera: Gripopterygidae): Insights into Morphological Variation and Molecular Species Delimitation.

Authors

Felipe Ribeiro Pereira Sarmento, Tácio Duarte, Ana Clara Pereira Teixeira, Frederico Falcão Salles

Published in

Neotropical entomology. Volume 54. Issue 1. Pages 96. Sep 10, 2025. Epub Sep 10, 2025.

Abstract

This study addresses historical uncertainties regarding morphological variation in the paraprocts of Tupiperla illiesi, a stonefly with a complex taxonomic history. We tested whether these variations represent phenotypic plasticity or distinct species using integrative taxonomy. Adult gripopterygids were collected from Estação Biológica de Boracéia utilizing Malaise and light traps. The morphology of the specimens was analyzed in accordance with existing literature, and selected individuals underwent DNA extraction, amplification, and sequencing of the cytochrome c oxidase subunit I (COI) barcode region. Molecular distances were estimated using the Kimura 2-parameter model, and clustering was determined using Neighbor-Joining and Bayesian methods. Species delimitation was further refined using the SPdel pipeline. The combined analysis of COI sequence and morphological differences in the paraprocts led to the identification of distinct morphotypes within T. illiesi, resulting in the description of a new species, Tupiperla tucum sp. nov.

PMID:
40931279
Bibliographic data and abstract were imported from PubMed on 11 Sep 2025.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.