Tianma modulates proteins with various neuro-regenerative modalities in differentiated human neuronal SH-SY5Y cells.

Authors

Umamaheswari Ramachandran, Arulmani Manavalan, Husvinee Sundaramurthi, Siu Kwan Sze, Zhi Wei Feng, Jiang-Miao Hu, Klaus Heese

Published in

Neurochemistry international. Volume 60. Issue 8. Pages 827-36. Epub Mar 28, 2012.

Abstract

Tianma (Rhizoma gastrodiae) is the dried rhizome of the plant Gastrodia elata Blume (Orchidaceae family). As a medicinal herb in traditional Chinese medicine (TCM) its functions are to control convulsions, pain, headache, dizziness, vertigo, seizure, epilepsy and others. In addition, tianma is frequently used for the treatment of neurodegenerative disorders though the mechanism of action is widely unknown. Accordingly, this study was designed to examine the effects of tianma on the proteome metabolism in differentiated human neuronal SH-SY5Y cells to explore its specific effects on neuronal signaling pathways. Using an iTRAQ (isobaric tags for relative and absolute quantitation)-based proteomics research approach, we identified 2390 modulated proteins, out of which 406 were found to be altered by tianma in differentiated human neuronal SH-SY5Y cells. Based on the observed data, we hypothesize that tianma promotes neuro-regenerative signaling cascades by controlling chaperone/proteasomal degradation pathways (e.g. CALR, FKBP3/4, HSP70/90) and mobilizing neuro-protective genes (such as AIP5) as well as modulating other proteins (RTN1/4, NCAM, PACSIN2, and PDLIM1/5) with various regenerative modalities and capacities related to neuro-synaptic plasticity.

PMID:
22710396
Bibliographic data and abstract were imported from PubMed on 15 Sep 2025.

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