Authors
Houria Benhamiche, Habiba Zerizer, Faiza Boughachiche, Zahra Azzouz, Mohammed Messaoudi
Published in
FEMS microbiology letters. Sep 12, 2025. Epub Sep 12, 2025.
Abstract
The process of solid-state fermentation (SSF) offers a sustainable platform for enzyme production, particularly proteases with industrial relevance. In this study, protease synthesis was investigated using a thermotolerant Streptomyces sp. strain C8, isolated from arid soil of Tamanrasset, Algeria. The strain, Gram-positive with biverticillate spore chains, grew optimally at 45°C, pH 7, tolerated 5% NaCl, and exhibited 93.21% 16S rRNA similarity to Streptomyces griseorubens NBRC 12780 (GenBank accession PV834194), suggesting novelty. Under SSF, various agro-industrial wastes were tested, with spent brewing grains emerging as the most promising substrate. Initial optimization via the one-factor-at-a-time (OFAT) approach identified incubation time, temperature, pH, and moisture as critical parameters, yielding maximum activity (82 U/mL) under 30°C, pH 7, four days, and 75% humidity. Further optimization using response surface methodology of Box-Behnken design (RSM) confirmed significant model performance (F = 8.47, p = 0.0003, R² = 0.9081), highlighting moisture, incubation time, and pH as key factors. The refined conditions (31°C, 55% moisture, three days, and pH 7) resulted in 128.70 U/mL activity. Overall, these findings demonstrate that low-cost agro-industrial byproducts, particularly spent brewing grains, can serve as efficient, eco-friendly substrates for protease production using thermotolerant Streptomyces strains, offering promising applications in biotechnology.
PMID:
40966658
Bibliographic data and abstract were imported from PubMed on 19 Sep 2025.
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