Resin infiltration and in-office dental bleaching on different substrates: hydrogen peroxide penetration, color change, and enamel morphology- an in vitro study.

Authors

Taynara S Carneiro, Bruno Baracco, Michel Wendlinger, Michael Willian Favoreto, Gabriel Cochinski, Gabrielle Centenaro, Laura Ceballos, Alessandro D Loguercio

Published in

Clinical oral investigations. Volume 29. Issue 10. Pages 465. Sep 19, 2025. Epub Sep 19, 2025.

Abstract

To evaluate the penetration of hydrogen peroxide into the pulp chamber, color change, and enamel morphology of human teeth with different substrates (sound and white spot), subjected to the application of a resin infiltration (RI) and in-office bleaching at different time intervals (immediately and 7 days after RI application).
Fifty-four sound human premolars were randomly distributed into six groups (n = 9), all subdivided according to substrate (sound and white spot): bleaching with 35% hydrogen peroxide only (HP); RI followed by immediate bleaching (RI/HPi); and RI followed by bleaching after 7 days (RI/HP7d). The concentration of hydrogen peroxide (µg/mL) was measured by UV-Vis spectrophotometry, color change was evaluated using a digital spectrophotometer (ΔE₀₀ and WI_D), and enamel surface morphology was analyzed by scanning electron microscopy (SEM) (α = 0.05).
In the groups without RI (HP), teeth with white spot lesions showed approximately 40% greater hydrogen peroxide penetration than sound teeth (p = 0.15). RI significantly reduced this penetration, regardless of the substrate or bleaching time (p > 0.02). According to WI_D values, the groups treated with RI (RI/HPi and RI/HP7d) achieved a final color similar to that of sound enamel treated with 35% HP. Enamel morphology was more homogeneous in the groups that received resin infiltration.
The application of RI prior to in-office bleaching significantly reduced hydrogen peroxide penetration into the pulp chamber without compromising bleaching efficacy. In teeth with white spot lesions, it led to a final shade comparable to that of sound teeth and resulted in a more uniform enamel morphology.
Resin infiltration is an effective, minimally invasive approach for treating white spot lesions, improving bleaching outcomes and limiting hydrogen peroxide penetration.

PMID:
40970989
Bibliographic data and abstract were imported from PubMed on 19 Sep 2025.

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