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Effect of Omega 3 supplementation during pregnancy and lactation on cognitive functions in rat offspring.

Created on 20 Sep 2025

Authors

Hande Nur Onur Öztürk, Perim Fatma Türker

Published in

PloS one. Volume 20. Issue 9. Pages e0319457. Epub Sep 19, 2025.

Abstract

This study investigates the effects of omega-3 supplementation starting in the preconception period, during the 1st, 2nd, and 3rd weeks of pregnancy, and lactation on the cognitive functions of rat offspring.
The study involved 30 mother rats divided into a test group receiving 400 mg/kg/day omega-3 supplementation via oral gavage and a control group with no supplementation. Omega-3 supplementation began at different stages for each group and continued through the lactation period (21 days). Two male pups from each mother, for a total of 60 pups, were tested for cognitive function at 45 days using the Morris Water Maze to assess spatial learning and reference memory.
Significant differences were found between groups in initial weights, pre-mating weights, pre-birth weights, number of pups, and gestation duration (p < 0.05), but not in weight gain during pregnancy (p > 0.05). The time to find the platform on test days 1 and 2 was significantly different between groups (p < 0.05). Differences in platform-finding times across trials were also significant (p < 0.05). However, no significant difference was found in the probe test on day 5 (p > 0.05).
Initiating omega-3 supplementation during the early stages of pregnancy may be more effective for both short-term and long-term memory.

PMID:
40971848
Bibliographic data and abstract were imported from PubMed on 20 Sep 2025.

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