A signal-amplifying electrochemical immunosensor based on palladium decorated CoS₂ hollow nanoboxes and gold-functionalized Fe₃O₄ for neuron-specific enolase detection.

Authors

Dehao Jia, Yujian Guo, Lei Yang, Xing Gao, Dongquan Leng, Qin Wei

Published in

Mikrochimica acta. Volume 192. Issue 10. Pages 685. Sep 20, 2025. Epub Sep 20, 2025.

Abstract

A sandwich-type electrochemical (EC) immunosensor for neuron-specific enolase (NSE) detection was constructed utilizing a signal amplification strategy. This strategy was implemented by utilizing palladium decorated CoS₂ hollow nanoboxes (CoS₂-Pd HNBs) as the signal indicator and gold-functionalized Fe₃O₄ (Fe₃O₄-Au) as the EC sensing platform. CoS₂ HNBs with hollow structures were synthesized via template method, which exhibited excellent electrocatalytic performance toward H₂O₂. Moreover, Pd nanoparticles (NPs) were decorated on CoS₂ HNBs by in situ reduction method, which further improved the electrocatalytic performance for H₂O₂ reduction due to the synergetic catalysis. Modifying Au NPs uniformly on Fe₃O₄ surface could not only enhance the electroconductivity of the EC sensing platform but also immobilize the capture antibody (Ab₁) via strong coordination. Fe₃O₄-Au nanocomposites were anchored to the magnetic glass carbon electrode because of good magnetic properties, improving stability of the EC immunosensor. Prominently, the well-established sandwich-type EC immunosensor with NSE as a targeted detection object exhibited a low detection limit of 0.29 pg/mL (S/N = 3) and a linear range from 500 fg/mL to 50 ng/mL. This work may provide a promising detection method for other biomarkers.

PMID:
40974402
Bibliographic data and abstract were imported from PubMed on 20 Sep 2025.

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