Preparation of 6-Azido-2-Chloropurine-2'-Deoxyriboside by Enzymatic Transglycosylation Reaction Catalyzed by Lactobacillus leichmannii Type II Nucleoside Deoxyribosyltransferase.

Authors

C S Alexeev, M A Konkina, N N Kurochkin, M S Drenichev

Published in

Doklady. Biochemistry and biophysics. Sep 21, 2025. Epub Sep 21, 2025.

Abstract

6-azido-2-chloropurine-2'-deoxyriboside, a valuable precursor for the preparation of modified 2-chloropurine nucleosides substituted at the 6-position of the heterocyclic base, was obtained by enzymatic transglycosylation. 6-azido-2-chloropurine-2'-deoxyriboside can also be used as a photocross-linking agent to study the nucleic acids-proteins interactions. A type II nucleoside deoxyribosyltransferase from Lactobacillus leichmannii was used as a biocatalyst. The optimal conditions for the formation of 6-azido-2-chloropurine-2'-deoxyriboside using 7-methyl-2'-deoxyguanosine as a carbohydrate residue donor were determined.

PMID:
40991171
Bibliographic data and abstract were imported from PubMed on 24 Sep 2025.

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