OsFLN2 gene knockout reduces leaf water status and photosynthetic performance of rice plants under water deficit.

Authors

Danyel Fernandes Contiliani, Simone Ferreira da Silva, Rafael Vasconcelos Ribeiro, Vitor Nolasco Moraes, Laísa Medeiros Rocha, Greice Lubini, Paula Macedo Nóbile, Tiago Campos Pereira, Silvana Creste

Published in

BMC plant biology. Volume 25. Issue 1. Pages 1266. Oct 01, 2025. Epub Oct 01, 2025.

Abstract

Abiotic stresses significantly impact plant growth and productivity. To overcome these challenges, plants employ photosynthetic acclimation mechanisms, enabling them to sustain photosynthetic efficiency under adverse conditions. The OsFLN2 gene has been identified as a key player in chloroplast development and response to heat and salinity stress. However, the physiological effects of OsFLN2 gene knockout and its impact on drought tolerance remain elusive. In this study, we explored the function of OsFLN2 in rice plants under water deficit conditions using a non-transgenic CRISPR knockout mutant. Our findings demonstrate that Osfln2 mutant plants exhibit higher sensitivity to water deficit, characterized by reduced photosynthetic activity and reduced transcriptional responsiveness of plastid genes, such as RbcL and PsaA. As a result, these plants display compromised carboxylation efficiency and reduced leaf water use efficiency at the maximum water deficit. Concluding, our results demonstrate the role of OsFLN2 on physiological acclimation of rice plants under water-limiting conditions.

PMID:
41034703
Bibliographic data and abstract were imported from PubMed on 02 Oct 2025.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.