Ultra-stable copper nanoclusters modulated by chitosan and the application to serum cholesterol detection.

Authors

Wenxiang Xiao, Yunqin Hu, Liuwen Shao, Jiannan Liu, Dong Wang, Hua Li

Published in

Mikrochimica acta. Volume 192. Issue 11. Pages 752. Oct 23, 2025. Epub Oct 23, 2025.

Abstract

Ultra-stable copper nanoclusters were fabricated for the sensitive determination of cholesterol based on fluorescence turn-off. Red-emitting copper nanoclusters capped with cysteine were first synthesized with a short storage time (15 d). Then, the addition of chitosan and the adjustment of media pH were applied to modulate the unstable clusters into exceptionally stable aggregates with cyan-green emission possessing a long lifetime over 180 days. The aggregation-induced emission (AIE) of chitosan-modified copper nanoclusters (Cys-CuNCs) can be significantly quenched by cholesterol due to the interaction between cholesterol and chitosan. The fluorescence response of Cys-CuNCs to cholesterol exhibits a rapid equilibrium (1 min), high sensitivity with a detection limit of 19.63 μmol·L⁻¹ (3σ, n = 3), and a wide linear concentration range of 25.0 ~ 1000.0 μmol·L⁻¹. The proposed method has good stability and can be employed for cholesterol determination in biofluids.

PMID:
41131412
Bibliographic data and abstract were imported from PubMed on 24 Oct 2025.

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