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Insect-Mediated Metabolic Activation of Etofenprox Amplifies Hidden Aquatic Toxicity in Rice Ecosystems.

Created on 31 Oct 2025

Authors

Huihui Zhang, Yixi Zhang, Jingting Wang, Jiaqi Zhang, Chuting Shi, Zewen Liu

Published in

Environmental science & technology. Oct 30, 2025. Epub Oct 30, 2025.

Abstract

Pyrethroid insecticides, though effective against pests, often exhibit high toxicity to aquatic organisms. A notable exception is etofenprox, which is uniquely approved for rice paddies due to its low aquatic toxicity. However, this study uncovers a hidden ecological risk: etofenprox undergoes metabolic activation in the insect pest Nilaparvata lugens via oxidation mediated by cytochrome P450 CYP425A1. The oxidation converts the etofenprox ether bond into an ester (α-CO, 2-(4-ethoxyphenyl)-2-methylpropyl 3-phenoxybenzoate), a hallmark of potent pyrethroids. This transformation dramatically amplifies aquatic toxicity, with α-CO exhibiting 23-79-fold higher lethality in fish and crabs than the parent compound, reaching levels comparable to those of conventional pyrethroids like cypermethrin. Our findings challenge the traditional structure-toxicity paradigms by uncovering a dynamic "metabolic activation-toxicity amplification" mechanism. Crucially, this bioactivation creates an indirect exposure pathway in integrated rice-fish-crab systems, where predators ingest toxic metabolite-laden pests, escalating ecological risks. This study reveals insect-mediated metabolic conversion as a critical driver of hidden aquatic toxicity. This finding redefines pesticide risk assessment by advocating for mandatory metabolite toxicity evaluation alongside parent compounds. Our results urge a reconsideration of etofenprox's safety in aquatic agroecosystems, with broader implications for pyrethroid design and unforeseen ecological risks.

PMID:
41167979
Bibliographic data and abstract were imported from PubMed on 31 Oct 2025.

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