Authors
Yuqi Peng, Si Long, Yuqing Zhong, JiaJia Chen, Zhi Chen, Xin Ju, Liangzhi Li
Published in
International journal of biological macromolecules. Pages 151545. Mar 20, 2026. Epub Mar 20, 2026.
Abstract
Lignocellulosic monosaccharides are valuable substrates for the production of high value-added chemicals. An enzymatic cascade was previously developed to convert two main biomass monosaccharides, D-xylose and d-glucose synergistically and produce rare sugars of D-xylulose and D-arabitol. However, the conversion of D-xylose was less than 80%, resulting in low reaction efficiency and tedious separation issues. In this study, the reaction was reevaluated from the viewpoint of enzyme adaption, and the isomerization of D-xylose was conducted by glucose isomerases instead of ribose-5-phosphate isomerase B to overcome the limitation of conversion. Under fine-tuned reaction conditions, glucose isomerases from Streptomyces rubiginosus (SrGI) and Piromyces sp. (PsGI) were combined with the arabitol dehydrogenase from Gluconobacter sp. JX-05 (GsArDH) and glucose dehydrogenase from Bacillus megaterium IWG3 (BmGDH), to convert D-xylose completely at 100 mM. In comparison, SrGI performed better than PsGI in the cascade system and the reaction was finished in 2 h. However, higher substrate concentrations led to uncompleted conversion of D-xylose due to substrate inhibition. This study demonstrated the feasibility of systematic refinement of lignocellulosic monosaccharides, achieved complete conversion of D-xylose, and provided insights relevant to the utilization potential of biomass lignocellulose.
PMID:
41865939
Bibliographic data and abstract were imported from PubMed on 23 Mar 2026.
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