Authors
Michael A Miller, Nicole Vo, Utkarsh, Ivan Sokirniy, Justin Pritchard, Benjamin S Freedman, Scott H Medina
Published in
Advanced science (Weinheim, Baden-Wurttemberg, Germany). Pages e20402. Jun 15, 2026. Epub Jun 15, 2026.
Abstract
Efficient delivery of gene editing ribonucleoproteins (RNPs) into the interior of solid tissues remains a key hurdle to the clinical translation of non-viral CRISPR-Cas9 technologies. Here, we report acoustically-actuated peptide nanoemulsions (NPeps) that can be spatiotemporally guided and activated by ultrasound to ballistically deliver RNPs into cells within the bulk of dense 3D cellular structures. Using human kidney organoids as a model, we demonstrate NPep vectors improve the spatial profile of gene editing in the organoid mass relative to commercial lipofection reagents, without disruption of tissue structure or qualitative viability features. This technologic paradigm is poised to advance imaging-guided, deep tissue RNP delivery modalities to expand the clinical diagnostic and therapeutic potential of CRISPR-Cas9 editing strategies.
PMID:
42295796
Bibliographic data and abstract were imported from PubMed on 15 Jun 2026.
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