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STAT1 itaconation prevents macrophage cytolistic mtDNA -induced inflammation in wear particle-induced aseptic loosening.

Created on 16 Jun 2026

Authors

Yifan Yu, Taihe Liu, Chenhao Pan, Haopeng Sun, Zhipeng Chen, Haoxian Liu, Wingcheuk Ko, Siyuan Tan, Jiankai Luo, Shixun Li, Changchuan Li, Yue Ding

Published in

Cell communication and signaling : CCS. Volume 24. Issue 1. Apr 16, 2026. Epub Apr 16, 2026.

Abstract

Prosthetic wear particle-driven macrophage inflammation severely limits the long-term efficacy of total joint replacements by causing aseptic loosening. However, the specific mechanisms by which wear particles induce macrophage inflammation remain incompletely elucidated. Itaconic acid produced by the Krebs cycle is markedly up-regulated in TiPs-stimulated macrophages, which may modulate mitochondrial metabolism through protein itaconation or competitive inhibition. Here, using 4-OI, we demonstrate that itaconate functions as an endogenous metabolic regulator that suppresses SDH activity, thereby significantly inhibiting STING pathway activation. Moreover, 4-OI can alkylate STAT1, preventing its phosphorylation and relieving transcriptional repression of the mitochondrial transcription factor TFAM, which stabilizes mitochondrial homeostasis and attenuates macrophage inflammation. In a murine calvarial osteolysis model, 4-OI reversed bone destruction induced by TiPs and TFAM knockdown. Collectively, our findings establish itaconate as a critical endogenous metabolite that alleviates wear particle–mediated inflammation and osteolysis by reprogramming macrophage metabolism.
The online version contains supplementary material available at 10.1186/s12964-026-02874-4.

PMID:
41992317
Bibliographic data and abstract were imported from PubMed on 16 Jun 2026.

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