Prognostic and therapeutic implications of BRAF mutations in acute myeloid leukemia.

Authors

Darren Lee, Yazan Abu-Shihab, Kate Plas, Deedra Nicolet, Krzysztof Mrózek, Mark J Routbort, Keyur P Patel, Christopher J Walker, Jill Buss, Andrew R Stiff, Andrea Laganson, Courtney D DiNardo, Naval G Daver, Tapan M Kadia, Farhad Ravandi, Andrew J Carroll, Jonathan E Kolitz, Bayard L Powell, William G Blum, Maria R Baer, Guido Marcucci, Geoffrey L Uy, Wendy Stock, Richard M Stone, L Jeffrey Medeiros, John C Byrd, James S Blachly, Robert L Bowman, Jeffrey W Tyner, Sanam Loghavi, Ann-Kathrin Eisfeld, Linde A Miles

Published in

Leukemia. Jun 15, 2026. Epub Jun 15, 2026.

Abstract

Mutations in the RAS/MAPK signaling pathway are recurrent in acute myeloid leukemia (AML), primarily involving NRAS and KRAS. In contrast, mutations in the gene encoding an effector protein, BRAF, occur at relatively lower frequencies in AML and are associated with poor outcomes. To date, no comprehensive analysis has assessed the clinical and molecular characteristics of BRAF-mutated AML. In this study, we report the identification of canonical and non-canonical BRAF mutations in ~1% of 5779 consecutive clinically and molecularly fully annotated AML patients treated at two major United States Cancer Centers (50/5779 AML patients: 21 newly diagnosed AML; 9 relapsed/refractory; 20 newly diagnosed secondary AML). We performed single-cell multiomic analysis on a subset of AML samples. BRAF mutations were enriched in myelodysplasia-related AML (AML-MR), and most mutations were located outside the V600 hotspot. Single-cell multiomic profiling delineated BRAF mutation class-specific patterns of co-mutations, clonality, and immunophenotypes. Notably, BRAF mutations and other signaling co-mutation(s) could be found in the same cell, a finding that significantly diverges from prior studies of RAS-mutant AML. In this cohort, BRAF-mutant AML patients had poor overall survival with currently available treatments, including venetoclax-based regimens. Drug sensitivity data suggest possible avenues for targeted treatment of BRAF-mutated AML.

PMID:
42297916
Bibliographic data and abstract were imported from PubMed on 16 Jun 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.