Authors
Alessandra Cardoso da Silva, Antonio Sergio Varela Junior, Raqueli Teresinha França, Anthony Valverde, Luciano da Silva Pinto, Carine Dahl Corcini
Published in
Cell biochemistry and function. Volume 44. Issue 6. Pages e70247.
Abstract
Lectins are carbohydrate-binding proteins that recognize specific glycan patterns on cell surfaces and have been increasingly explored as analytical tools in reproductive biology. This study characterized the interaction of a lectin isolated from Abelmoschus esculentus seeds (AEL) with ovine spermatozoa and evaluated its compatibility with sperm functional parameters during short-term refrigeration. Eighteen ejaculates were diluted in a Tris-egg yolk extender supplemented with 0, 1, or 3 µg/mL AEL and stored at 5°C for 24 h. Sperm motility (CASA) and flow cytometry were used to assess plasma membrane integrity and fluidity, mitochondrial membrane potential, and reactive oxygen species production. Confocal microscopy was performed to determine the localization pattern of AEL binding. AEL labeling was predominantly restricted to the sperm midpiece region. Supplementation with AEL at the tested concentrations did not affect motility patterns, membrane integrity, mitochondrial membrane potential, or ROS levels after 24 h of storage (p > 0.05). These findings indicate that AEL exhibits a consistent midpiece-associated binding pattern and does not compromise sperm functional parameters under refrigerated conditions. The results support the use of AEL as a compatible probe for investigating glycan distribution in the midpiece region of ram spermatozoa.
PMID:
42299477
Bibliographic data and abstract were imported from PubMed on 16 Jun 2026.
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