Protocol for expressing and purifying recombinant full-length Tau by combining affinity chromatography with preparative HPLC.

Authors

Ruiheng Jing, Sayanta Mahapatra, Abhishek Bimali, Morgane Herlory, Maciej A Walczak

Published in

STAR protocols. Volume 7. Issue 3. Pages 104617. Jun 16, 2026. Epub Jun 16, 2026.

Abstract

Deposits of microtubule-associated protein full-length Tau (Tau2N4R) are implicated as a hallmark of Alzheimer's disease. Its biochemical and structural characterization is key to understanding disease progression, aggregate toxicity, and designing therapeutics. We present a protocol for expressing and purifying Tau2N4R by combining affinity chromatography with preparative high-performance liquid chromatography (HPLC). We describe steps for linking an N-terminal hexahistidine tag and a cleavable SUMO tag to Tau2N4R. This purification ensures an ultrapure Tau2N4R that gives rise to heparin-induced and cofactor-free in vitro fibrillation.

PMID:
42301817
Bibliographic data and abstract were imported from PubMed on 17 Jun 2026.

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