A comparative evaluation of apical sealing ability of NeoPutty, Biodentine, and Bio-c repair using a radioactive isotope method - An in vitro study.

Authors

Srishti Rathore, Jagat Bhushan, Amandeep Singh Uppal, Ruchi Singla, Vijiyata Dani Chadha, Satvika Sharma

Published in

Journal of conservative dentistry and endodontics. Volume 29. Issue 5. Pages 552-557. Epub Apr 30, 2026.

Abstract

Treatment of teeth with immature apices is difficult to manage by conventional endodontics. Apical barrier formation using bioceramic materials offers a biologically favorable solution, providing a hermetic seal that supports periapical healing and facilitates successful root canal treatment.
The aim is to evaluate and compare the sealing ability of NeoPutty, Biodentine, and Bio-C repair used as apical plugs in teeth with immature apex using radioactive isotopes.
Sixty-nine extracted single-rooted human teeth were decoronated at the CEJ, leaving a root segment of 15 mm. Further, 3 mm of the apex was resected. Standardizing 12 mm root length, biomechanical preparation was carried out, and a divergent retrograde preparation using a NeoEndo orifice opener (30/08) was done to simulate an immature root apex. Samples were randomly distributed into 3 experimental groups (n = 23): Group 1 (NeoPutty), Group 2 (Biodentine), and Group 3 (Bio-C Repair) with an apical plug of 4 mm and two controls, Group 4 (Negative) and Group 5 (Positive). After 4 days, the teeth were immersed in 99TcNaO₄ for 4 h, and microleakage was assessed using a gamma-ray spectrometer.
Data normality was assessed using the Shapiro-Wilk test, which showed a nonnormal distribution. Therefore, nonparametric tests were applied: Kruskal-Wallis ANOVA for intergroup comparisons and Mann-Whitney U test for pairwise analysis.
The highest percentage of radioactivity was observed in Group 5: 71%, followed by Group 2: 55%, Group 3: 53%, Group 1: 48%, and Group 4: 4%.
Neoputty exhibited the best apical sealing ability.

PMID:
42306596
Bibliographic data and abstract were imported from PubMed on 17 Jun 2026.

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