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Genome Wide Identification and Characterization of BrE2F Family Gene of Brassica rapa.

Created on 18 Jun 2026

Authors

Naima Sultana, Md Jahid Hasan Jone, Anjan Chandra Sharma, Setu Rani Saha, Mohammad Rashed Hossain, Shirin Akhter, Md Anisur Rahman, Jobadatun Naher

Published in

International journal of genomics. Volume 2026. Pages 7106391. Epub Jun 15, 2026.

Abstract

The E2F family gene is one of the most important G1-S checkpoint regulatory transcription factors to monitor and regulate the accurate DNA replication and progression of the cell cycle in eukaryotes. Expression of this gene in multiple environmental factors has been reported in diversified plant species. However, characterization and investigation of the putative influential role of this family gene in Brassica species were not reported. In addition to expression profiling and mRNA target analysis, we have comprehensively identified and characterized 14 BrE2F/DP transcription factors from the data in available databases of Brassica rapa and compared them with five other species. Six pairs of segmentally duplicated BrE2F/DP paralogous genes were identified in this study, with Ka/Ks values of < 1.0. The findings indicated their evolution through segmental duplication across the chromosome after the Brassica splits from Arabidopsis and functional similarities with Brassica oleracea proteins. Most of the stress-responsive microRNA (miRNA) targeted BrE2F/DP5, BrE2F/DP12, BrE2F/DP1, and BrE2F/DP8. Cis-element composition and diversified functional expressions indicated their involvement in biological pathways for adaptation under multiple stress resistances, including cell cycle progression during growth and development. The findings provided comprehensive information on the multifunctional roles of the E2F/DP family in Brassica spp., importantly, biotic and abiotic stress resistance.

PMID:
42312093
Bibliographic data and abstract were imported from PubMed on 18 Jun 2026.

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