Sphingobium trunci sp. nov. and Peribacillus folii sp. nov.: two novel phosphate- and zinc-solubilizing bacteria isolated from Kaempferia marginata Carey in Thailand.

Authors

Tanakwan Budsabun, Pattaraporn Yukphan, Wattana Panphut, Wongsakorn Phongsopitanun, Somboon Tanasupawat, Kanchana Sitlaothaworn

Published in

International journal of systematic and evolutionary microbiology. Volume 76. Issue 6.

Abstract

Two endophytic bacterial strains, designated as Sx8-8ᵀ and SI8-4ᵀ, were isolated from the stem and leaf tissues, respectively, of Kaempferia marginata Carey collected in Thailand. A comprehensive taxonomic investigation employing a polyphasic approach was conducted to characterize these strains. Strain Sx8-8ᵀ is a Gram-negative, aerobic, rod-shaped, non-spore-forming bacterium that produces yellow pigment on Reasoner's 2A agar (R2A) medium. Phylogenetic analysis based on 16S rRNA gene sequences placed this strain within the genus Sphingobium, showing the highest similarity (98.5%) to Sphingobium chungbukense KACC 12347ᵀ (=DJ77^T). The draft genome was 4.7 Mb in size with a G+C content of 64 mol%. Genome-wide comparisons with closely related type strains yielded an average nucleotide identity based on blastn (ANIb) and MUMmer (ANIm) values of 82.2-84.0% and 86.7-86.9%, respectively, with digital DNA-DNA hybridization (dDDH) values of 28.4-29.1%. Chemotaxonomic data supported its affiliation to the genus, with Q-10 identified as the major respiratory quinone, along with characteristic fatty acid and polar lipid profiles. These findings support the proposal of a novel species, Sphingobium trunci sp. nov., with Sx8-8ᵀ (=LMG 33805ᵀ=TBRC 17746ᵀ) as the type strain. Strain SI8-4ᵀ is a Gram-positive, aerobic, spore-forming, rod-shaped bacterium, producing pale pink pigment on R2A medium. It was identified as a member of the genus Peribacillus, sharing the highest 16S rRNA gene sequence similarity (99.0%) with Peribacillus frigoritolerans JCM 11681ᵀ and Peribacillus muralis DSM 16288ᵀ. The genome was 4.6 Mb in size, with a G+C content of 43 mol%. Comparative genomic analyses revealed ANIb and ANIm values of 80.0-86.0% and 84.3-87.8%, respectively, while dDDH values were 24.3-32.1%. The major quinone was MK-7, and the fatty acid and polar lipid compositions were consistent with members of the genus Peribacillus. Based on genotypic and phenotypic distinctiveness, strain SI8-4ᵀ is proposed to represent a novel species, Peribacillus folii sp. nov. The type strain is SI8-4ᵀ (=KACC 23903ᵀ=KCTC 43756ᵀ=TBRC 17747ᵀ).

PMID:
42313453
Bibliographic data and abstract were imported from PubMed on 18 Jun 2026.

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