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Cyclometalated Anionic Benzimidazole-Based Luminescent Iridium Complexes with Tunable Organelle-Specific Cell Imaging.

Created on 19 Jun 2026

Authors

Paramita Jana, Chand Husen Ansary, Nabashree Chakraborty, Ahmad Husain, Apurba L Koner, Srikanta Patra

Published in

Inorganic chemistry. Jun 18, 2026. Epub Jun 18, 2026.

Abstract

Herein, we present an example of a neutral [1] and a cationic [2] heteroleptic cyclometalated iridium complex incorporating benzimidazole-based ligands, where the NN benzimidazole ligand coordinates in its anionic form. The formation and molecular integrity of the complexes were confirmed by HRMS and multinuclear NMR spectroscopy, while single-crystal X-ray crystallography verified the neutral nature of [1] and the anionic coordination of the NN ligand. Complex [2] is water-soluble, whereas [1] shows reduced solubility in most solvents. Both complexes are luminescent, exhibiting better emission quantum yields and lifetimes (∼1.0 μs) in water than other solvents. Density functional theory (DFT) studies show that the HOMO is mainly localized on the iridium center and the NN benzimidazole ligand, while the LUMO is primarily localized on the CN benzimidazole ligand with minimal iridium contribution. Spin density analysis indicates that the emission originates from 3MLLCT states. Both complexes show moderate cytotoxicity (IC50 ∼ 15 μM) in HeLa and B16F10 cancer cells and CHO normal cells. Additionally, the cellular imaging of the complexes in HeLa cells revealed that complex [1] has specific localization in the endoplasmic reticulum (ER), whereas complex [2] has specific localization in mitochondria, demonstrating their potential as organelle-specific phosphorescent probes for bioimaging.

PMID:
42314109
Bibliographic data and abstract were imported from PubMed on 19 Jun 2026.

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