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A prospective case series of autologous protein solution injection in knee osteoarthritis: two-year outcomes stratified by structural severity and inflammatory phenotype.

Created on 19 Jun 2026

Authors

Shinji Satake, Kei Sakao, Nagakazu Shimada

Published in

The Knee. Volume 62. Pages 104549. Jun 18, 2026. Epub Jun 18, 2026.

Abstract

Two-year durability of autologous protein solution (APS) in knee osteoarthritis stratified by Kellgren-Lawrence (KL) grade and inflammatory phenotype, and its role as a non-operative bridge for patients deferring total knee arthroplasty (TKA), remain uncharacterised.
Single-arm prospective case series of 67 knees (KL 2-4) receiving a single APS injection with 2-year follow-up.
VAS, KOOS Pain/ADL, OMERACT-OARSI responder rate, and Kaplan-Meier TKA-free survival. Patients were stratified by KL grade and by pre-injection synovial fluid volume (<10 vs ≥10 mL) as an index of joint inflammation. KL grade had independent dual assessment with adjudication (κ = 0.615). Subgroup analyses were exploratory.
Of 67 knees, 43 (64.2%) completed 2-year PROM; TKA status was confirmed in 57 (85.1%). VAS and KOOS Pain improved significantly versus baseline at every timepoint. In KL4 (n = 17; all initially recommended for TKA), median VAS improved from 50 to 28 mm at 1 year and 30 at 2 years; KOOS ADL showed a ceiling effect. Two-year Kaplan-Meier TKA-free survival was 94.0% (95% CI 88.4-99.7%); no KL4 patient underwent TKA within 24 months (log-rank p = 0.252). The OMERACT-OARSI responder rate was 70.3%. Within KL3, patients with ≥10 mL synovial fluid showed greater VAS improvement at 6 months (p = 0.030); similar direction was seen at other timepoints but did not reach significance. No serious APS-related adverse events occurred.
APS was associated with sustained pain reduction over 2 years and high observed TKA-free survival. Absence of randomisation precludes causal inference; findings are hypothesis-generating.

PMID:
42314238
Bibliographic data and abstract were imported from PubMed on 19 Jun 2026.

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