Progresses of transposon research in Saccharomyces cerevisiae.

Authors

Wenbo Lin, Yu Lv, Yuhao Zhang, Zhaoxia Jin, Linxia Liu, Dawei Zhang

Published in

Synthetic and systems biotechnology. Volume 14. Pages 487-497. Epub Jun 11, 2026.

Abstract

Transposons are mobile genetic elements that have attracted sustained scientific interest since their discovery in maize, evolving into indispensable tools in genetic research. Saccharomyces cerevisiae serves as a preferred model for transposon studies, benefiting from a mature genetic manipulation system, a well-characterized genome, and cellular mechanisms that resemble those of higher organisms. This review summarizes recent advances and application strategies of transposons in S. cerevisiae. We begin by classifying transposons based on their transposition mechanisms, with a focus on representative systems including the Tn, hAT, Ty, piggyBac (PB), Tc1/mariner and CRISPR-associated transposon (CAST) families, detailing their molecular mechanisms. We further discuss the applications of endogenous and engineered transposons in yeast, address key challenges such as insertion bias and genomic instability, and summarize corresponding engineering strategies aimed at enhancing their efficiency and specificity. Our goal is to provide a roadmap for developing transposons into efficient, precise, and low-off-target genome-editing tools, thereby advancing the construction of yeast cell factories.

PMID:
42318494
Bibliographic data and abstract were imported from PubMed on 19 Jun 2026.

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