Authors
Ayda Aray, Arezoo Morshedi, Reihaneh Ramezani, Nader Shokoufi
Published in
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. Volume 363. Issue Pt 1. Pages 128259. Jun 14, 2026. Epub Jun 14, 2026.
Abstract
Bovine milk-derived exosomes are biocompatible nanovesicles with significant potential for drug delivery, yet reliable methods for their label-free identification remain limited. This study employs fluorescence spectroscopy to characterize the intrinsic optical fingerprint of BMEs. Initially, the Excitation-Emission Matrix served as an exploratory tool to map the spectral landscape. Second-derivative analysis was then applied to resolve intrinsic fluorophore hotspots obscured by background interference, followed by spectral deconvolution to separate overlapping components. This analysis revealed a distinct multi-component fluorescence signature arising from Tryptophan, NAD(P)H, AGE-related MRPs, Porphyrins, Flavins (Riboflavin/FAD), Vitamin A, and Schiff-base-type fluorophores. Moreover, all these autofluorescence spectra were recorded at pH 3, 7, and 10, revealing characteristic pH-dependent spectral changes for each component, most notably the emergence of a well-resolved 515 nm peak at pH 3 confirming riboflavin's neutral-to-anionic speciation, as well as a red-shift in porphyrin emission wavelength at pH 3 and 7. Collectively, these intrinsic optical signatures validate BMEs as self-reporting nanocarriers, providing a robust, non-invasive biosensing baseline for quality control and tracking without the structural compromise associated with external tagging.
PMID:
42320163
Bibliographic data and abstract were imported from PubMed on 20 Jun 2026.
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