Authors
Feng Huang, Li Li, Yi Zhou, Xinyi Peng, Rong Liu, Zhengjun Cheng
Published in
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. Volume 363. Issue Pt 1. Pages 128269. Jun 16, 2026. Epub Jun 16, 2026.
Abstract
Employing a dual-ligand surface engineering strategy, red-fluorescent copper nanoclusters (Neu5Ac@5-MTU-CuNCs) was fabricated via an eco-friendly one-pot method with 5-methyl-2-thiouracil (5-MTU) and N-acetylneuraminic acid (Neu5Ac) as ligands. The incorporation of Neu5Ac can improve the coordination environment, restrict the vibration of 5-MTU on the CuNCs surface, and simultaneously create steric hindrance between its rigid sugar ring structure and the pyrimidine moiety of 5-MTU. Based on this, the fluorescence intensity of Neu5Ac@5-MTU-CuNCs at 680 nm was boosted, along with improved detection sensitivity for vitamin B12 (VB12) and diosmetin (Dio). Detection limits of 0.039 μM (VB12) and 0.160 μM (Dio) were received, with linear ranges of 0.10-52.25 μM and 0.50-62.25 μM. Meanwhile, synthesis conditions of Neu5Ac@5-MTU-CuNCs and corresponding quenching mechanisms were intensively studied. The CuNCs was successfully employed to monitor VB12/Dio in various foods with good recoveries, demonstrating high reliability. A compact and economical paper-based strip was formulated by leveraging Neu5Ac@5-MTU-CuNCs, enabling expeditious, equipment-free, and visual semi-quantitative determination of VB12/Dio for field deployment. Besides, the Neu5Ac@5-MTU-CuNCs also held promise for applications in multifunctional anti-forgery and dactyloscopic analysis.
PMID:
42320162
Bibliographic data and abstract were imported from PubMed on 20 Jun 2026.
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