Targeted Plasma Amino Acid Metabolic Remodeling and Pharmacological Regulation in Heart Failure Rats Using PRISE-NUS-bs-HSQC 2D NMR Spectroscopy.

Authors

Renliang Xue, Xindan Zhang, Yating Ge, Qingce Zang, Ruiping Zhang, Yinghong Wang

Published in

NMR in biomedicine. Volume 39. Issue 8. Pages e70334.

Abstract

Nuclear magnetic resonance (NMR) metabolomics offers a robust platform for the analysis of complex biological samples, but its application is often constrained by signal overlap and limited sensitivity. In this study, we developed an optimized two-dimensional NMR approach (probe-induced sensitivity enhancement with nonuniform sampling and band-selective ¹H-¹³C HSQC [PRISE-NUS-bs-HSQC]) to enable high-throughput, high-resolution semiquantitative profiling of 22 amino acids in plasma from a rat model of heart failure (HF). When combined with spatially resolved metabolomics of cardiac tissue using AFADESI-MSI, plasma levels of valine, leucine, isoleucine, phenylalanine, methionine, and glutamine showed positive correlations with metabolic disturbances in the infarct (I) area of the heart. A composite biomarker panel derived from these amino acids distinguished HF rats from controls with an area under the curve (AUC) of 0.926. Using multiple therapeutic strategies, including traditional Chinese medicine (TCM), Western medicine, combined therapy with TCM and Western medicine, and active ingredients from Chinese medicine, we observed that all interventions reversed HF-associated amino acid metabolic dysregulation to varying extents. Notably, combined treatment with Qishen Yiqi dropping pills and sacubitril/valsartan produced broader metabolic normalization and more pronounced synergistic effects than either monotherapy. Collectively, this study establishes a noninvasive and efficient 2D NMR-based framework for exploring the association between systemic amino acid metabolism and regional cardiac injury in HF. The integrated analytical strategy provides mechanistic insight into amino acid metabolic remodeling during HF progression and underscores its translational potential for noninvasive diagnosis and therapeutic evaluation.

PMID:
42322080
Bibliographic data and abstract were imported from PubMed on 20 Jun 2026.

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