Authors
Maike Däther, Elsa Peev, Annika Fröhlich, Binje Vick, Sogol Fatourechi, Gilles Gasparoni, Matthias Heiss, Corinna C Pleintinger, Emmanuel Asu Bisong, Hans Hurmiz, Davide Guglielminotti, Yasmin V Gärtner, Tina Aumer, Karsten Spiekermann, Jörn Walter, Irmela Jeremias, Franziska R Traube, Thomas Carell
Published in
Angewandte Chemie (International ed. in English). Pages e6265286. Jun 21, 2026. Epub Jun 21, 2026.
Abstract
Ten-eleven translocation (TET) enzymes are critical epigenetic regulators, which oxidize the methylated cytosine nucleobase 5-methyl-dC (mdC) in the genome to 5-hydroxymethyl-dC (hmdC) in an α-ketoglutarate-dependent manner. Because the presence of mdC in the promoter region of a given gene silences its expression, this oxidation goes in hand with the reactivation of such silenced genes. In different highly aggressive cancers such as acute myeloid leukemia (AML) and glioblastoma, loss of TET enzyme function, and therefore reduced hmdC levels pave the way for tumor development. Impairment of TET activity can occur through metabolic inhibition, through loss-of-function mutations in TET genes themselves, and finally through suppression of TET-expression via epigenetic silencing. Reactivation of TET enzyme expression represents a major aim of epigenetic cancer therapy. Here we show that the carbocyclic antimetabolite 5-aza-2'deoxycytidine (cAzadC), which is supposed to suppress the methylation of DNA during replication, leads to a substantial increase of TET2 expression and strongly increasing hmdC levels. We show that the treatment with cAzadC goes in hand with the broad reactivation of the cellular antitumor responses. With patient-derived xenograft AML-mouse models, we show that this translates into a strongly improved anticancer effect in vivo.
PMID:
42323802
Bibliographic data and abstract were imported from PubMed on 22 Jun 2026.
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