Authors
Mengjuan Zhu, Yu Shi, Ming Geng, Xun Zhang, Qinggang Xu
Published in
Analytical methods : advancing methods and applications. Jun 22, 2026. Epub Jun 22, 2026.
Abstract
Fluoroquinolones (FQs) are widely used in animal husbandry for the prevention of a variety of animal diseases. However, residues of FQs in animal products that exceed safe limits may pose a serious threat to human health. Therefore, the development of colloidal gold immunochromatographic test strips with high specificity and high sensitivity is crucial for the accurate detection and management of such antibiotic residues. In this study, a monoclonal antibody (mAb) with high specific reactivity to FQs was prepared, which exhibited cross-reactivity with more than 10 fluoroquinolones. The mAb showed that the half-maximal inhibitory concentration (IC50) for PEF was 0.023 ng mL-1, with a linear range from 0.0103 to 0.069 ng mL-1. A competitive enzyme-linked immunosorbent assay (ic-ELISA) was established. The recoveries of PEF ranged from 80% to 120%, and the average coefficient of variation was below 10%. Based on this mAb, an immunochromatographic test strip for the detection of FQ residues in actual milk samples was developed. The results demonstrated that the test strip could simultaneously detect 12 fluoroquinolones, with a LOD of 1-10 ng mL-1. This study highlights that establishing a method capable of simultaneous broad-spectrum screening and high-sensitivity detection is essential for effectively monitoring fluoroquinolone residues and ensuring consumer safety.
PMID:
42325063
Bibliographic data and abstract were imported from PubMed on 22 Jun 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 7
- Comments 0