Authors
Yufeng Guo, Chunpeng Feng, Mengchuan Lai, Meichen Liu, Yalin Xi, Lei Yin, Meiyun Shi
Published in
Analytical methods : advancing methods and applications. Jun 22, 2026. Epub Jun 22, 2026.
Abstract
Azoxystrobin is a widely used strobilurin fungicide. Its environmental persistence and potential toxicity to aquatic organisms demand accurate trace-level quantification in biological tissues. However, its sensitive and specific determination in small tissue samples using conventional methods remains challenging. In this study, a novel high-sensitivity analytical method based on ultra-performance liquid chromatography coupled with triple-stage mass spectrometry (UPLC-MS3) was established for the trace determination of azoxystrobin in zebrafish liver tissues. Following protein precipitation extraction, chromatographic separation was performed on a C18 column using a gradient of 0.1% aqueous formic acid and acetonitrile. Detection relied on an optimized MS3 transition (m/z 404.0 → 371.9 → 344.2). The method exhibited excellent linearity (r > 0.9984) from 0.1 to 20 ng mL-1, with accuracy between -3.33% and 3.67% and precision (CV) between 5.57 and 10.19%. Consistent recoveries (94.93-106.64%) and minimal matrix effects (99.45-104.79%) were achieved across all tissue matrices. Compared to conventional MRM, MS3 scanning significantly enhanced specificity by reducing endogenous interference. The validated approach was successfully applied to tissue distribution studies in zebrafish, confirming its reliability for environmental toxicology research and providing a robust platform for investigating fungicide biodistribution in aquatic organisms.
PMID:
42324935
Bibliographic data and abstract were imported from PubMed on 22 Jun 2026.
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