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In vitro and in silico evaluation of anthelmintic potential of Ficus hispida L.f. Leaf extracts targeting β-tubulin.

Created on 22 Jun 2026

Authors

Arunpandiyan Jeyakumar, Sardar Khan Shamsheeth Khan, Brito Raj Selvaraj, Jayalakshmi B, Bhavya E

Published in

Natural product research. Pages 1-13. Jun 21, 2026. Epub Jun 21, 2026.

Abstract

Helminth infections remain a major global health burden, and growing resistance to existing anthelmintic drugs necessitates the development of novel therapeutic agents. The present study evaluated the anthelmintic potential of Ficus hispida L.f. leaf extracts using integrated phytochemical, in vitro, and in silico approaches. Leaf extracts prepared by Soxhlet extraction and cold maceration using solvents of varying polarities were tested against Pheretima posthuma, with albendazole as the reference drug. The ethanolic Soxhlet extract induced rapid paralysis in 2.98 ± 0.17 min and death in 5.31 ± 0.07 min at 100 mg/mL. GC-MS analysis identified several pharmacologically relevant compounds. Molecular docking against β-tubulin demonstrated strong binding affinities, particularly for 9-Phenanthrenemethyl 2,6-dimethylbenzoate (-6.872 kcal/mol), followed by Thebaol (-6.135 kcal/mol) and 2-(1-Phenyl ethylidene-hydrazono)-3-methyl-2,3-dihydrobenzothiazole (-5.850 kcal/mol), suggesting microtubule disruption as a possible mechanism of action. These findings support F. hispida as a promising source of plant-derived anthelmintic agents.

PMID:
42324881
Bibliographic data and abstract were imported from PubMed on 22 Jun 2026.

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