Protocol for mitochondrial DNA extraction, purity evaluation, and global methylation assessment via LC-MS/MS.

Authors

Argyri Chroni, Theodora Alexiou, John Zafeiropoulos, Vasiliki Chondrou, Argiris Symeonidis, Argyro Sgourou

Published in

STAR protocols. Volume 7. Issue 3. Pages 104643. Jun 22, 2026. Epub Jun 22, 2026.

Abstract

Established epigenetic mechanisms, including nuclear (n)DNA methylation, are also known to regulate the mitochondrial (mt) genome despite its lack of histones. However, studies referring to evaluations of mtDNA methylation are often influenced by technical and biological confounders. We present an optimized protocol for extracting mtDNA from peripheral blood and bone marrow mononuclear cells, followed by steps for qualitative assessment to exclude nDNA contaminations and a downstream quantitative analysis of the mtDNA global methylation profile using liquid chromatography combined with mass spectrometry.

PMID:
42329770
Bibliographic data and abstract were imported from PubMed on 23 Jun 2026.

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