LncRNA Gas5 directs SUV39H2 to establish heterochromatin and maintain genome stability.

Authors

Juntao Liu, Jiani Yang, Wen Ye, Dandan Zhang, Nan Su, Hong Wang, Yanping Zhang, Shaorong Gao, Lan Kang

Published in

Protein & cell. Jun 23, 2026. Epub Jun 23, 2026.

Abstract

Constitutive heterochromatin, characterized by histone H3 lysine 9 trimethylation (H3K9me3), is essential for genome stability, pluripotency, and developmental fidelity. While the SUV39H histone methyltransferases catalyze H3K9me3 deposition, their locus-specific targeting mechanisms remain unclear. Here, via RNA depletion or RNA-binding affinity mutation, we establish that RNA binding is indispensable for SUV39H2 recruitment to chromatin and H3K9me3 maintenance in mouse embryonic stem cells (ESCs). Through RNA immunoprecipitation sequencing (RIP-Seq) and functional validation, we identify the long non-coding RNA Gas5 as a specific SUV39H2 interactor. Depletion of Gas5 or disruption of the SUV39H2-RNA interaction leads to the loss of self-renewal capacity of ESCs, abolishes H3K9me3 enrichment, along with profound genomic instability, mitotic errors, and γH2AX foci accumulation. These findings reveal a critical lncRNA-dependent mechanism governing the SUV39H2-H3K9me3 axis, which directly couples RNA metabolism to the preservation of pluripotency and genome integrity in stem cells.

PMID:
42334949
Bibliographic data and abstract were imported from PubMed on 24 Jun 2026.

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