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Advances in Interleukin-2 Engineering and Delivery Systems for Cancer Immunotherapy.

Created on 24 Jun 2026

Authors

Xiaolei Du, Yujie Chen, Mengchen Xu, Hongyun Liu, Jiafei Liu, Feihe Ma, Linqi Shi

Published in

ACS applied bio materials. Jun 24, 2026. Epub Jun 24, 2026.

Abstract

Interleukin-2 (IL-2) is a multifunctional cytokine that plays a central role in the proliferation, differentiation, and function of regulatory T cells (Tregs), effector T cells, and natural killer (NK) cells. Given the immunomodulatory properties of IL-2, high-dose IL-2 has been approved for the treatment of metastatic renal cell carcinoma and melanoma, making it the first cytokine-based immunotherapy to achieve clinical translation. However, the extremely short half-life in vivo, significant systemic toxicity, and immune suppression mediated by the preferential activation of Tregs severely limit its clinical application and therapeutic efficacy. In recent years, with a deeper understanding of the composition and distribution of IL-2 receptor subunits and their downstream signaling pathways, protein engineering research aimed at improving the therapeutic index of IL-2 has become increasingly active. This article reviews the structural-functional relationships of IL-2 and its receptor complexes, summarizes engineering strategies for IL-2 variants, and examines IL-2 delivery systems designed to achieve selective targeting of effector cells in cancer immunotherapy. These approaches have made progress in mitigating IL-2-induced systemic toxicity, providing a design basis for the development of safer and more effective IL-2 therapies.

PMID:
42340732
Bibliographic data and abstract were imported from PubMed on 24 Jun 2026.

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